Monday, 7 October 2013

Mouse Monoclonal Antibody



                 There are many uses for antibodies in therapeutic and diagnostic research. This is possible by the number of ways in which the antibody and antigens are capable of interaction. The monoclonal antibody has an important tool in Molecular biology, Medicine and Biochemistry. This is used for the development of the lower cost production with special focus on experiment. These are mono specific antibodies, which are now identical in nature. This is a monoclonal type of antibody and the name of this mouse monoclonal antibody is c-Myc Antibody (9E10). Its main host is a mouse and it is at present in purified form. They are produced by a single parent cell which has one type of B-lymphocytes. The clone of this antibody is 9E10 and mouse IgG1 Isotype with the quantity of 200µg/ml. This monoclonal reacts on Rat, Human, Primate, Mouse and their genes are Myc[AU016757, bHLHe39, Myc2] and MYC[ bHLHe39,MRTL].

Monday, 30 September 2013

ANTIBODY PRODUCTION



Antibody is the form of protection which protect against a specific disease by receiving the vaccinated human or animal. Antibodies are host proteins which are produced by our immune system in response to antigens that enter the body. Antibody production has both meanings general and specific. Antibody production refers to the creating a usable specific antibody, which includes the step of immunogen preparation, hybridoma creation, collection, screening, isotyping in a particular method. The Antibody produces in response to immunization which used as a critical component of a non-animal laboratory test. Additional antigen injections are given when the level of antibody is not adequate for research purposes. For safe injection into the laboratory or farm animals, the antibody production involves antigen samples for preparation to evoke antigen-specific antibodies in the serum by the high expression levels then which can be recovered from the animal. It successfully depends upon carefully planning, synthesize the target antigen, and choose an appropriate immunogenic protein.

Sunday, 22 September 2013

Antibody reagents



An antibody reagent is an antibody that is used in an experiment to know the specific molecular target of interest for detection and isolation of that particular molecule. Antibody reagents are prepared through an antibody production process which helps in recognizing a specific molecular antigen of interest or another target material containing unspecified antigen. Antibodies recognize a molecular target. The main characteristic of an antibody reagent is that its role is recognized in an experiment through a process of binding an epitope on a molecular antigen. This process also helps to know the role of antigen which has been recognized. It is necessary to recognize the role of the experimental antigen. This process is used in the preparation of many effective remedies that are effective against particular groups of antigens. This modern technique has been used in order to find remedial solution for many harmful diseases.